FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_90.35100000045a7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_90.35100000045a7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81082
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC68978.50620359635924No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA15131.8660121851952343No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11811.4565501590981969No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10771.328284946103944No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA9261.1420537233911348No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8791.0840877136725784No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.9817222071483189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6780.8361905231740707No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT5460.6733923682198268RNA PCR Primer, Index 41 (95% over 21bp)
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA5190.6400927456155497No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4990.6154263585012704No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4320.5327939616684344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.46989467452702205No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3690.45509484225845437No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC3450.4254951777213191RNA PCR Primer, Index 34 (95% over 23bp)
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3200.3946621938284699No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3090.3810956809156163No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2990.3687624873584766No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2560.31572975506277595No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2410.2972299647270664No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2250.2774968550356429No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2190.2700969389013591No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2110.26023038405564736No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT2100.2589970646999334RNA PCR Primer, Index 41 (95% over 23bp)
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.2466638711427937No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1870.2306307195185121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1780.21953084531708641No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1720.21213092918280257No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA1640.20226437433709085No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA1640.20226437433709085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT1620.1997977356256629No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1610.19856441626994892No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.19733109691423495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT1510.18623122271280926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1500.1849979033570953No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1450.17883130657852545No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1380.17019807108852766No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1350.16649811302138576No Hit
GTACGGGAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.1652647936656718No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA1300.1603315162428159No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.14799832268567623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1180.1455316839742483No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG1170.14429836461853432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1160.14306504526282035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT1130.13936508719567844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT1130.13936508719567844No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA1130.13936508719567844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1110.1368984484842505No Hit
GTGGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAA1110.1368984484842505No Hit
GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1110.1368984484842505No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAA1090.1344318097728226No Hit
GGTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1080.1331984904171086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTT970.11963197750425496No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA960.11839865814854099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT960.11839865814854099No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT950.11716533879282702RNA PCR Primer, Index 34 (95% over 22bp)
CCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG950.11716533879282702No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA940.11593201943711304No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG940.11593201943711304No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.11469870008139907No Hit
GAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA930.11469870008139907No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC920.1134653807256851No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA900.11099874201425718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGT900.11099874201425718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT890.10976542265854321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT850.10483214523568733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT840.10359882587997336No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA830.1023655065242594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7350.039.79209545
ATGCCGT8050.036.33191744
ACCCCTC250.00209658635.99654442
ACTCGAC8200.035.66730527
CCACGAG8200.035.66730520
CACGAGA8200.035.66730521
ACGAGAC8200.035.66730522
TCTCGTA8150.035.6100838
CATCTCG8100.035.55214336
ACGGCAT8100.035.55214332
GGCATCT8050.035.4934934
GCATCTC8050.035.4934935
GTATGCC8250.035.4511442
TATGCCG8250.035.4511443
TCGTATG8250.035.4511440
CGAGACT8250.035.4511423
AGACTCG8250.035.4511425
GACTCGA8250.035.4511426
CTCGTAT8250.035.4511439
CGACGGC8150.035.33403430
Produced by FastQC (version 0.11.2)