FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_9.3510000004505d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_9.3510000004505d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34452
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC1034930.03889469406711RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT11223.256704980842912No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG2970.8620689655172413No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT2510.7285498664809009No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC2510.7285498664809009RNA PCR Primer, Index 45 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT970.28155114362010913RNA PCR Primer, Index 45 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG470.13642168814582606No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGAT206.9570955E-444.9753277
TGCCGTC11250.041.5771945
ATGCCGT12550.037.2703944
TCTCCGA12900.036.43350211
AATCTCG12850.036.40026536
ATCTCCG12850.036.40026510
TCCGAGC12850.036.40026513
TCTCGTA12800.036.36676838
CTCGTAT12800.036.36676839
ACGAGAC12900.036.31188222
GAGACGT12900.036.31188224
CTCCGAG12950.036.2928312
AGCCCAC12850.036.27791617
GCCCACG12850.036.27791618
CCCACGA12850.036.27791619
GAGCCCA12900.036.2591816
CCGAGCC12900.036.2591814
ATCTCGT12850.036.22526637
TATGCCG12850.036.22526643
CGTATGC12850.036.22526641
Produced by FastQC (version 0.11.2)