FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_89.35100000045a53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_89.35100000045a53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19694
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC701135.59967502792728No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT6033.061846247588098RNA PCR Primer, Index 41 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG1890.9596831522291053No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT1540.7819640499644561RNA PCR Primer, Index 41 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC330.16756372499238348RNA PCR Primer, Index 34 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8000.043.59375445
ATGCCGT8800.039.6306844
CGAGCCC9150.038.36065715
AGCCCAC9100.038.32417317
GTATGCC9100.038.32417342
GCCCACG9100.038.32417318
CCACGAG9100.038.32417320
TATGCCG9100.038.32417343
GAGCCCA9100.038.32417316
CACGAGA9100.038.32417321
CCCACGA9100.038.32417319
ACGAGAC9100.038.32417322
CCGAGCC9100.038.32417314
TCTCCGA9100.038.32417311
CGAGACT9100.038.32417323
GAGACTC9100.038.32417324
CTCCGAG9100.038.32417312
CGTATGC9050.038.2872941
TCGTATG9050.038.2872940
CTCGTAT9050.038.2872939