Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_88.35100000045a46.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 49855 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC | 2340 | 4.69361147327249 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT | 291 | 0.5836927088556815 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT | 268 | 0.5375589208705245 | No Hit |
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT | 235 | 0.47136696419616886 | No Hit |
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC | 155 | 0.3109016146825795 | Illumina PCR Primer Index 1 (95% over 22bp) |
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC | 105 | 0.21061077123658611 | No Hit |
TATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTT | 63 | 0.12636646274195165 | Illumina PCR Primer Index 1 (95% over 21bp) |
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGAT | 53 | 0.106308294052753 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGAACCT | 20 | 6.968473E-4 | 45.0 | 1 |
TACGGCT | 20 | 6.968473E-4 | 45.0 | 22 |
GGCTTAT | 20 | 6.968473E-4 | 45.0 | 25 |
CTTATTA | 20 | 6.968473E-4 | 45.0 | 27 |
CGGCTTA | 20 | 6.968473E-4 | 45.0 | 24 |
ATACGGC | 25 | 3.8371843E-5 | 45.0 | 21 |
TCTGGAC | 25 | 3.8371843E-5 | 45.0 | 35 |
TGATAAT | 30 | 1.1243322E-4 | 37.500004 | 7 |
GATTTGT | 30 | 1.1243322E-4 | 37.500004 | 45 |
AATTGAT | 30 | 1.1243322E-4 | 37.500004 | 16 |
ATTATTC | 30 | 1.1243322E-4 | 37.500004 | 30 |
GAATTGA | 30 | 1.1243322E-4 | 37.500004 | 15 |
CTGATAA | 30 | 1.1243322E-4 | 37.500004 | 6 |
CCTGATA | 30 | 1.1243322E-4 | 37.500004 | 5 |
ACTTAGA | 25 | 0.0020878722 | 36.0 | 40 |
GACTTAG | 25 | 0.0020878722 | 36.0 | 39 |
ACGGCTT | 25 | 0.0020878722 | 36.0 | 23 |
AACCTGA | 25 | 0.0020878722 | 36.0 | 3 |
GAACCTG | 25 | 0.0020878722 | 36.0 | 2 |
CTTAGAT | 25 | 0.0020878722 | 36.0 | 41 |