FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_88.35100000045a46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_88.35100000045a46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49855
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC23404.69361147327249No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT2910.5836927088556815No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT2680.5375589208705245No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT2350.47136696419616886No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC1550.3109016146825795Illumina PCR Primer Index 1 (95% over 22bp)
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC1050.21061077123658611No Hit
TATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTT630.12636646274195165Illumina PCR Primer Index 1 (95% over 21bp)
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGAT530.106308294052753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCT206.968473E-445.01
TACGGCT206.968473E-445.022
GGCTTAT206.968473E-445.025
CTTATTA206.968473E-445.027
CGGCTTA206.968473E-445.024
ATACGGC253.8371843E-545.021
TCTGGAC253.8371843E-545.035
TGATAAT301.1243322E-437.5000047
GATTTGT301.1243322E-437.50000445
AATTGAT301.1243322E-437.50000416
ATTATTC301.1243322E-437.50000430
GAATTGA301.1243322E-437.50000415
CTGATAA301.1243322E-437.5000046
CCTGATA301.1243322E-437.5000045
ACTTAGA250.002087872236.040
GACTTAG250.002087872236.039
ACGGCTT250.002087872236.023
AACCTGA250.002087872236.03
GAACCTG250.002087872236.02
CTTAGAT250.002087872236.041