FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_87.35100000045a2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_87.35100000045a2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331445
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC61641.859735401046931No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT10210.3080450753518683No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7550.039.03698345
ATGCCGT8350.035.29691744
CGTATGC8600.034.27084441
TATGCCG8600.034.27084443
CACGAGA8500.034.14980721
CCCACGA8600.034.00923519
GCATGAT8800.034.0084232
GAGACTA8800.033.75271624
GTATGCC8750.033.68334642
GACTAAG8900.033.3734726
ACTAAGC8850.033.30776627
TCGTATG8850.033.30274240
CTCGTAT8900.033.11564639
GATCTCG8900.033.11564636
GCCCACG8900.032.86285418
AGCATGA9150.032.7075531
ATCTCGT9100.032.3878337
TCTCGTA9200.032.03578638
CCACGAG9300.031.449420
CTAAGCA9450.031.43109928
Produced by FastQC (version 0.11.2)