FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_85.351000000459e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_85.351000000459e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255337
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC50291.9695539620188223No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT13170.5157889377567685No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3200.1253245710570736No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2810.11005063895949276No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC207.0238713E-444.9988258
TCGCGCG207.0238713E-444.9988259
GGGGCTA207.0238713E-444.99882536
TGCCGTC5400.041.24892445
CGCGCGC250.002104316135.9990610
GCGCCAT250.002104316135.9990621
AAGCGCC250.002104316135.9990619
AGCGCCA250.002104316135.9990620
GGCTAGT250.002104316135.9990638
ATGCCGT6850.032.51740344
CGCGCTT352.814706E-432.14201712
TATGCCG7050.031.5949243
GTATGCC7000.031.49917642
CGAGACT7100.031.37242323
CACGAGA7150.031.15303221
CGTATGC7050.030.95663841
GACTAAG7200.030.93669326
CCCACGA7100.030.73863619
CTCGTAT7150.030.52367839
ACGAGAC7300.030.51290122
Produced by FastQC (version 0.11.2)