FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_84.351000000459cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_84.351000000459cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409596
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC74341.8149591304602581No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT12590.30737604859422457No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6700.1635758161700798No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT6440.15722809793064385No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA4760.11621207238351935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9550.041.46518345
ATGCCGT10500.037.4992944
CGTATGC10850.036.28963541
TATGCCG10800.036.2493143
GATCTCG10950.036.16369636
GTATGCC10950.035.9582242
TCGTATG11050.035.63280540
ACGAGAC11200.035.35647222
CGAGACT11300.035.04358323
CTCGTAT11300.034.8444739
CCCACGA11300.034.8444719
AAGCATG11400.034.73618330
AGCATGA11450.034.58449631
ACTAAGC11450.034.58449627
GACTAAG11450.034.58449626
GAGACTA11650.034.3770324
GCATGAT11600.034.33124532
CACGAGA11550.034.2850621
GCCCACG11500.034.23848318
TCTCGTA11500.034.23848338
Produced by FastQC (version 0.11.2)