FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_83.351000000459a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_83.351000000459a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590360
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC65801.1145741581407953No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT8730.14787587234907515No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG8390.14211667457144792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8200.038.41152641
GTATGCC8300.038.219842
ATGCCGT8350.037.99415644
CTCGTAT8300.037.94873439
TCGTATG8450.037.2750940
TATGCCG8600.036.88654743
GCCCACG8700.036.20396818
CGAGACT8800.035.79255723
TGCCGTC8900.035.64620245
ACTAAGC8650.035.63597527
CCCACGA8850.035.5903419
GATCTCG8750.035.4828436
TCTCGTA8950.035.4440638
AGCATGA8800.035.0315131
GAGACTA9000.034.99716624
GACTAAG8800.034.77285826
CACGAGA9100.034.61258321
CTAAGCA9050.033.81514728
ACGAGAC9600.033.04420522
GCATGAT9500.032.68710332