FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_82.3510000004598a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_82.3510000004598a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279044
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC161275.779375295652298No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA28191.0102349450265908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.7497025558693252No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA18350.6576023852869082No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT17760.6364587663594272No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17370.6224824758819397No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA16940.6070727197144536No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16680.597755192729462No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15310.5486589928470063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13630.4884534338670604No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA12590.451183325927094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.4393572339846046No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10690.3830937056521552No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10490.3759263772021617No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA9460.33901463568469486No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9460.33901463568469486No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7100.25444015997477104No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7020.25157322859477355No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.24548099941227908No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6760.242255701609782No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6250.22397901406229845No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.21430312065480714No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA5130.18384197474233455No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5060.1813334097848368No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4780.17129914995484583No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA4310.15445592809736097No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG4180.1497971646048652No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3870.13868780550737517No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT3760.13474577485987874No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA3510.12578661429738677No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC3430.12291968291738938Illumina PCR Primer Index 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT3390.12148621722739067No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA3310.11861928584739323No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3150.1128854230873984No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.11001849170740098No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3040.10894339243990195No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA2930.1050013617924055No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2890.1035678961024068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC19400.041.75224745
ATGCCGT20850.038.740744
TGATCTC21150.037.76565635
TATGCCG21300.037.71096443
GTATGCC21350.037.62265442
AGACTAA21600.037.60386725
GAGACTA21600.037.60386724
CTAAGCA21600.037.499728
TAAGCAT21600.037.499729
AAGCATG21550.037.482330
CACGAGA21600.037.39553521
ATCTCGT21300.037.39406637
CGTATGC21450.037.3423641
GACTAAG21700.037.3268926
GATCTCG21400.037.3244736
CTCGTAT21350.037.30649639
AGCATGA21700.037.22320631
CATGATC21650.037.2052533
GCATGAT21650.037.2052532
CCACGAG21650.037.2052520
Produced by FastQC (version 0.11.2)