FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_81.35100000045954.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_81.35100000045954.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16366
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC815449.82280337284614No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT7354.491017964071856No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG3292.0102651839178787No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT1060.6476842233899548No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATTCGTATGCCGTCT200.12220457045093486No Hit
CTTATACACATCTCCGAGCCACGAGACTAAGCATGATCTCGTATGCCGTCT180.10998411340584137No Hit
CTTATACAATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT170.10387388488329465No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATTATCTCGTATGCCGTC170.10387388488329465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9250.042.32432645
ATGCCGT10150.038.57142644
ATCTCCG10350.038.2608710
TCTCCGA10400.038.07692711
CCGAGCC10350.038.0434814
TCCGAGC10350.038.0434813
CTCCGAG10350.038.0434812
CGAGCCC10200.037.94117715
GAGCCCA10300.037.7912616
ACATCTC10600.037.783028
CATCTCC10550.037.7488149
CCCACGA10400.037.64423419
AGCCCAC10350.037.60869617
GCCCACG10350.037.60869618
CTAAGCA10550.037.53554528
ACGAGAC10500.037.49999622
AGCATGA10500.037.49999631
CGAGACT10500.037.49999623
AGACTAA10500.037.49999625
GAGACTA10500.037.49999624