FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_80.3510000004593a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_80.3510000004593a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374510
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC89182.3812448265733894No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT10130.2704867693786548No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG6600.17623027422498733No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5680.15166484206029213No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5260.14045018824597474No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC5110.13644495474086138No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT5020.13404181463779338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.122827160823476No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAT4470.11935595845237777No Hit
GCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATCG4120.11001041360711328No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT4070.10867533577207551No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA4030.1076072735040453No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA3990.10653921123601505No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG3810.10173293102987903No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT3800.10146591546287148No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3790.10119889989586392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT603.6379788E-1241.2524522
ACGGCTT659.094947E-1238.07918523
CTGGACT659.094947E-1238.07409736
ATGCCGT12050.036.781544
TATGCCG12200.036.32927343
CGTATGC12100.036.25763741
CTCGTAT12150.036.10843339
TGCCGTC12300.036.02910245
CATATGC250.002101926836.0117533
CCTATCG250.002104685236.00213626
GTGTCAT250.00210744635.99252311
TGGACCA12450.035.79003531
TCGTATG12300.035.66808740
CACGAGA12400.035.3804421
ATACGGC702.1827873E-1135.3545221
CCCACGA12300.035.29754619
GACTCTG12600.035.1806626
GCCCACG12400.035.01288618
ATCTCGT12550.034.9575737
GTATGCC12700.034.89898742
Produced by FastQC (version 0.11.2)