FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_79.35100000045910.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_79.35100000045910.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288681
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC44011.5245201450736279No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT9920.343631898185194No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5000.17320156158527925No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4810.16661990224503864No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA3720.12886196181944776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5200.041.10355445
CCTCCGA250.002105057735.99806244
ATGCCGT6200.034.11106544
GTATGCC6350.033.65960342
TATGCCG6300.033.5696243
CGTATGC6450.033.1377541
CTGGACC6500.032.88284330
CATCTCG6400.032.6935536
CGAGACT6550.032.6318323
CACGAGA6500.032.5367121
CCCACGA6550.032.28833819
TCTGGAC6500.032.19057529
TCGTATG6650.032.14112540
GACTCTG6700.031.90126426
ATCTCGT6700.031.56546237
GCCACTC501.975659E-631.49830438
TGGACCA6650.031.46446831
ACGAGAC6750.031.33164622
GGACCAT6700.031.22965832
CTCGTAT6850.031.20269839
Produced by FastQC (version 0.11.2)