FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_78.351000000458f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_78.351000000458f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343103
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC43831.2774589554740121No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA8110.23637216812444078No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT7370.21480430074933768No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT7350.21422138541487543No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA6950.20256307872563048No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG4780.1393167649364768No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT4300.1253267969093829No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCCG207.027259E-444.99883328
TGCCGTC4400.040.9080345
ATGCCGT5100.034.8520444
GGACCAT5200.034.18180532
TATGCCG5250.033.85626643
GTATGCC5250.033.85626642
GCCCACG5300.033.5368718
CGTATGC5300.033.5368741
CCCACGA5350.033.2234419
ACGAGAC5350.033.2234422
CTCGTAT5400.032.91581339
CACGAGA5400.032.91581321
CGAGACT5450.032.61383423
CATCTCG5400.032.4991636
TCGTATG5550.032.02619640
TCTCGTA5500.031.90826438
CTGGACC5500.031.90826430
GACCATC5550.031.62080233
ATCTCGT5800.030.64575837
TGGACCA5700.030.39394831