FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_77.351000000458db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_77.351000000458db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117500
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC13791.1736170212765957No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2270.19319148936170213No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC2030.1727659574468085No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG1500.1276595744680851No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT1370.11659574468085106No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA1290.1097872340425532No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA1230.1046808510638298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1600.040.7812545
CATATCA250.00209943536.0000044
CGCGCTT250.00209943536.00000412
GACTACT250.00209943536.00000424
AAAAAGT1750.033.4285741
GTCCTAA352.8050932E-432.1428571
ATGCCGT2050.031.82926844
CATCTCG2100.031.07142836
AAAAGTA1900.030.7894732
TATGCCG2200.030.6818243
GGACCAT2200.030.6818232
CACGAGA2350.030.63829821
TCCTAAA300.005128691429.9999982
CCCACGA2400.029.99999819
CGGGACG300.005128691429.99999817
CGAGACT2400.029.99999823
CCACGAG2400.029.99999820
CTGATCA300.005128691429.99999825
TCTGGAC2350.029.68085129
CTGGACC2350.029.68085130
Produced by FastQC (version 0.11.2)