FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_75.35100000045898.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_75.35100000045898.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402514
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC129253.2110684348867364No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT23470.5830853088339785No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6250.15527410226725033No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5210.12943649164997986No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG5140.12769742170458667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15400.041.48869745
ATGCCGT17450.036.61466644
CCGGGTT250.002105436.00029829
TATGCCG18300.034.91398643
CGTATGC18300.034.79104641
ACGAGAC18450.034.75638622
GTATGCC18400.034.60196742
GGACCAT18450.034.50819432
CCCACGA18600.034.47180619
CGAGACT18550.034.44772323
CTGGACC18600.034.3551230
CATCTCG18350.034.3327136
GCCCACG18700.034.28746818
CACGAGA18700.034.28746821
TCGTATG18450.034.2643240
ATCTCGT18550.034.0838437
TCTGGAC18700.034.05108629
GACCATC18650.034.0217333
ACTCTGG18850.033.89948727
TGGACCA19000.033.7460931
Produced by FastQC (version 0.11.2)