FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_74.3510000004587e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_74.3510000004587e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817734
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC61580.7530566174330529No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG9500.11617469739548557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT8300.034.42705544
TATGCCG8300.034.4249543
CGTATGC8350.034.21881541
GTATGCC8500.033.61495242
CACGAGA8850.033.0502221
TCGTATG8600.032.96247540
CTCGTAT8700.032.84219439
GCCCACG8800.032.7266518
CCCACGA9050.032.31982819
CGAGACT9300.030.9671523
CATCTCG9250.030.88941636
TCTGGAC9500.030.07837729
ACGAGAC9700.029.9221122
TGCCGTC9600.029.7650645
TCTCGTA9600.029.76323938
GACTCTG10000.029.02444626
GACGCAC554.165604E-628.6340687
TGGACCA10150.028.37384431
ATCTCGT10050.028.20669437
CTCTGGA10800.027.08281928
Produced by FastQC (version 0.11.2)