Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_73.35100000045855.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 42547 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC | 12633 | 29.691870167109315 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT | 1199 | 2.818060027734035 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT | 359 | 0.8437727689378804 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG | 298 | 0.7004019084776835 | No Hit |
ATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTTC | 267 | 0.6275413072602064 | Illumina PCR Primer Index 7 (95% over 22bp) |
TATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTT | 112 | 0.26323830117282065 | Illumina PCR Primer Index 7 (95% over 21bp) |
ATTGTAGCTTTTCTCCTCTTAAAAAACAACCTAAGAAACCAAAATTCCTCT | 94 | 0.22093214562718877 | No Hit |
ATTTGATTCGATTCGATCATTTGGATTCAATTTGTTACATTTCGATTCGAT | 48 | 0.11281641478835172 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCTTC | 30 | 2.1163924E-6 | 44.999996 | 45 |
TGCCGTC | 1430 | 0.0 | 41.695805 | 45 |
ATGCCGT | 1520 | 0.0 | 39.226974 | 44 |
AGCCCAC | 1560 | 0.0 | 38.365383 | 17 |
GCCCACG | 1560 | 0.0 | 38.365383 | 18 |
CACGAGA | 1560 | 0.0 | 38.365383 | 21 |
CGTATGC | 1555 | 0.0 | 38.34405 | 41 |
AGACTCT | 1555 | 0.0 | 38.34405 | 25 |
CTCGTAT | 1555 | 0.0 | 38.34405 | 39 |
TCGTATG | 1550 | 0.0 | 38.322582 | 40 |
GGACCAT | 1545 | 0.0 | 38.300972 | 32 |
GACCATC | 1545 | 0.0 | 38.300972 | 33 |
ATCTCGT | 1540 | 0.0 | 38.27922 | 37 |
CATCTCG | 1540 | 0.0 | 38.27922 | 36 |
TCTCGTA | 1540 | 0.0 | 38.27922 | 38 |
CCCACGA | 1565 | 0.0 | 38.242813 | 19 |
CTGGACC | 1565 | 0.0 | 38.242813 | 30 |
TGGACCA | 1565 | 0.0 | 38.242813 | 31 |
CTCTGGA | 1560 | 0.0 | 38.221153 | 28 |
GTATGCC | 1560 | 0.0 | 38.221153 | 42 |