FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_73.35100000045855.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_73.35100000045855.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42547
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC1263329.691870167109315No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT11992.818060027734035No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT3590.8437727689378804No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG2980.7004019084776835No Hit
ATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTTC2670.6275413072602064Illumina PCR Primer Index 7 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTT1120.26323830117282065Illumina PCR Primer Index 7 (95% over 21bp)
ATTGTAGCTTTTCTCCTCTTAAAAAACAACCTAAGAAACCAAAATTCCTCT940.22093214562718877No Hit
ATTTGATTCGATTCGATCATTTGGATTCAATTTGTTACATTTCGATTCGAT480.11281641478835172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC302.1163924E-644.99999645
TGCCGTC14300.041.69580545
ATGCCGT15200.039.22697444
AGCCCAC15600.038.36538317
GCCCACG15600.038.36538318
CACGAGA15600.038.36538321
CGTATGC15550.038.3440541
AGACTCT15550.038.3440525
CTCGTAT15550.038.3440539
TCGTATG15500.038.32258240
GGACCAT15450.038.30097232
GACCATC15450.038.30097233
ATCTCGT15400.038.2792237
CATCTCG15400.038.2792236
TCTCGTA15400.038.2792238
CCCACGA15650.038.24281319
CTGGACC15650.038.24281330
TGGACCA15650.038.24281331
CTCTGGA15600.038.22115328
GTATGCC15600.038.22115342