FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_72.3510000004583b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_72.3510000004583b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171496
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC40342.3522414516956665No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT12340.7195503102113169No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG2940.1714325698558567No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2500.14577599477538836No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT2330.13586322713066193No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG2080.1212856276531231No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2030.11837010775761533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4700.041.64326545
AGGTCGC250.002100516336.00566
CGCGCTT250.002103526835.99510212
ATGCCGT5750.034.03884544
ATAATAC401.5512092E-533.755253
CTGTCCA606.373739E-933.7454079
GTATGCC6100.032.45459742
TATGCCG6100.032.08579643
CGCTATG6200.031.94045330
CTCGCTA6300.031.43346228
TCGCTAT6350.031.18595329
ACGAGAC6350.031.18595322
GCTATGA6400.030.94231231
CGAGACT6400.030.94231223
ACTCGCT6450.030.7024527
CGTATGC6400.030.58177441
TCGTATG6400.030.58177440
GACTCGC6500.030.46627826
CTCGTAT6450.030.34470739
AGACTCG6550.030.2337125
Produced by FastQC (version 0.11.2)