FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_71.35100000045811.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_71.35100000045811.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233531
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC228239.77300658156733No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT40101.7171167853518374No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG10880.46589103802064824No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2810.12032663757702404No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG2700.1156163421558594No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAG2450.10491112528957611No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2430.10405470794027345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC24500.043.80320445
ACCCGCT301.1347179E-437.513576
ATGCCGT28950.036.99235544
CGCTATG29900.036.0427530
CCCGCAG250.002104367135.997631
GCTATGA29950.035.9825831
CTCGCTA30100.035.8780128
ACTCGCT30100.035.8780127
AGACTCG30400.035.8199825
TATGCCG29850.035.80163643
GATCTCG29850.035.80163636
CGAGACT30300.035.78969623
GTATGCC29900.035.74176842
TCGCTAT30250.035.700129
GACTCGC30300.035.6411926
ACGAGAC30450.035.6133922
TATGATC30200.035.6102133
GAGACTC30550.035.5704624
TGATCTC30050.035.56335435
CCCACGA30450.035.53950519