FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_7.35100000045026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_7.35100000045026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233873
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC3737115.979185284320977No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT99544.256156118919242RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG18010.7700760669252116No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA15270.6529184642947241No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12490.534050531698828No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10990.4699131579960064No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT9770.4177480940510448RNA PCR Primer, Index 44 (95% over 23bp)
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9130.3903828146045076No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA8910.38097599979476043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.3801208348120561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA7540.3223971984795167No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA7040.30101807391190943No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5460.2334600402782707No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5270.22533597294257995No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC5170.22106014802905852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.21849465308094565No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC4910.20994300325390278No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4810.2056671783403813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.2043844308663249No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC4580.19583278103928198No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4070.17402607398032266No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA3800.16248134671381478No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3570.15264694941271545No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG3560.1522193669213633No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3430.14666079453378542No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG3330.14238496962026398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG3310.1415298046375597No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA3180.1359712322499818No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3150.13468848477592538No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.13126782484510824No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA2970.1269919999315868No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2840.12143342754400892No Hit
AAAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG2770.11844035010454391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC40450.043.05192645
TAGATCG250.002101772236.006674
TGTCCAA250.0021039835.99897423
ATGCCGT50100.034.7145844
TATGCCG52800.032.93940443
CTCGTAT52750.032.88531539
TCTCGTA52550.032.88203438
ATCTCGT52700.032.8311337
TCGTATG52850.032.82309740
TATCTCG52650.032.81957636
CGTATGC53050.032.78417641
GTATGCC53050.032.78417642
ATATCTC53050.032.6145335
CACGAGA53250.032.53428321
GAGACCC53250.032.53428324
CCCTAGA53050.032.52970528
ACCCTAG53050.032.52970527
CCACGAG53400.032.5271620
GACCCTA53200.032.52256826
ACGAGAC53300.032.50376522
Produced by FastQC (version 0.11.2)