FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_69.351000000457dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_69.351000000457dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312489
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC96023.072748160735258No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT25690.8221089382346258No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG3910.12512440437903416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10950.042.7385145
CTACTAG504.840149E-836.0162661
GCTATAC250.00210340736.0047383
ATGCCGT13600.034.2453544
TATGCCG14100.032.87140743
CGCTATG13950.032.74100530
CGTATGC14200.032.63991541
CTCGCTA14050.032.50797328
CGAGACT14350.032.4555223
CTCGTAT14250.032.2096139
TCGTATG14250.032.2096140
GTATGCC14400.032.18658442
TCGCTAT14350.031.98515329
ACTCGCT14300.031.93965127
ACGAGAC14600.031.89977622
ATCTCGT14500.031.80944337
GCCCACG14650.031.79090318
CCCACGA14700.031.68277419
GATCTCG14500.031.65427436
GACTCGC14500.031.65427426
Produced by FastQC (version 0.11.2)