FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_68.351000000457cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_68.351000000457cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380308
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC154794.07012211155169No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT25510.6707721110258001No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG8410.2211365524785174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTGG207.026011E-445.00171315
TGCCGTC17350.043.70483445
ATGCCGT20050.037.8193944
TATGCCG20800.036.4557143
GTATGCC20800.036.4557142
TCGTATG20900.036.16886540
GACTCGC20900.035.95830526
CGTATGC21050.035.9111341
ACTCGCT20900.035.85064327
CGCTATG21100.035.5108330
CGAGACT21300.035.4943123
CTCGCTA21150.035.42687628
GATCTCG21350.035.30114736
AGACTCG21250.035.26016225
GCTATGA21350.035.20039731
TCGCTAT21300.035.17739529
CCCACGA21600.035.0013319
CTCGTAT21600.034.8925739
CTATGAT21550.034.8737132
CACGAGA21750.034.8633921
Produced by FastQC (version 0.11.2)