FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_66.3510000004577f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_66.3510000004577f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196518
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC32681.662951994219359No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT8720.4437252567194862No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA8570.4360923681291281No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA7380.37553811864562026No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT5600.28496117404003707No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT4440.22593350227460082No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2160.10991359570115715No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT1990.10126298863208458No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG1970.10024527015337018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3950.042.72151645
TATGCCG4700.035.90425543
GATCTCG4500.035.536
CGTATGC4700.035.42553341
GTATGCC4800.035.15625442
ATGCCGT4800.035.15625444
ACTCGCT4800.034.68750427
GACTCGC4800.034.68750426
ATCTCGT4700.034.46808637
CTCGTAT4700.034.46808639
CTCGCTA4850.034.329928
CGCTATG4800.034.21875430
TCGTATG4800.034.21875440
TCTCGTA4750.034.10526338
CGAGACT4900.033.9795923
CTGTCCA401.5540169E-533.759
CTATGAT4950.033.63636432
GCCCACG4950.033.63636418
GCTATGA4950.033.63636431
CACGAGA4950.033.63636421