H23LNBCXX_l01n01_bir_0113_pc_65.35100000045762.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pc_65.35100000045762.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74941
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC	14608	19.492667565151255	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT	1790	2.3885456559159874	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG	401	0.5350876022471011	No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCT	391	0.5217437717671235	No Hit
ATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCTTC	254	0.3389332941914306	Illumina PCR Primer Index 5 (95% over 21bp)
GTTGAAATTGTTTAAGAACCTTTCCTAAGAAAACAAGCAAGCAAGAAAGAA	137	0.18281047757569288	No Hit
ATGCACTTGTGTTCCATTTTTCAATTCAATTGTCACAGTTTCGTGACTAAG	123	0.16412911490372425	No Hit
TAAGATAACGGTACAATTTGGGTCTTTTACATGTGGCTACCCAGTTTTCTT	114	0.15211966747174444	No Hit
ATAATTAAGAGATCTGACATTCCGAGATTTACGAATGCTTTCTTCCTGTAG	112	0.14945090137574893	No Hit
TATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCTT	106	0.14144460308776238	No Hit
ATGGAATACTATGCAGCCATAAAAAATGATGAGTTCATGTCCTTTGTACAG	97	0.12943515565578254	No Hit
TGATTGAGTTTCTTTAAATCTGTCCAATCTCAAGTCATTTTTATTTGCCAT	89	0.1187600912718005	No Hit
ACTTCTTAGAAGATACGTGGTATTATCCTCATTGTACAGTTGAGCAAAGTA	88	0.11742570822380273	No Hit
AATGTAATCCATGTTGAAAGAGACAGAAAGGGAAAGAGAGGAAGGAAAGAA	86	0.11475694212780721	No Hit
AATGGATGTAGTTAAAGGAACCTTTTCTACAGGAGGACAATATCAAATTAT	86	0.11475694212780721	No Hit
TACAGTGGTAGCAAATTACAATTTGTGCATGTCTTGCATATTAGTATAAGG	86	0.11475694212780721	No Hit
AGTGGAAACTAAGTAGCAACTAAATATGAACTGTTACACCAAAACTGTTTA	85	0.11342255907980944	No Hit
CTTTTTGAATTTCTCTGATTGTTTTCTTTTGATCATCCTCGTTTTTATCTC	83	0.11075379298381392	No Hit
GATTCATAGAAGAGACAATATCCCATCCACTCACTTAGTATGATGTCAACT	81	0.10808502688781842	No Hit
GTTTATTGGGAGGACATATGAAATTTACCAAAAAGTTAGCTACTTTGGCAG	78	0.10408187774382513	No Hit
ATTCAAAACTATGTCAACTCACAATTCTAAAAACGAAGATTTCTTGCTTTC	77	0.10274749469582738	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGACA	20	6.9941516E-4	44.995995	33
AGTGTGG	20	6.9941516E-4	44.995995	8
GGAGGAC	20	6.9941516E-4	44.995995	32
TGCCGTC	1655	0.0	42.68502	45
ATGCCGT	1825	0.0	38.70888	44
CGTCTTC	35	6.1742085E-6	38.567993	45
CCCACGA	1855	0.0	37.961582	19
TCCGAGC	1870	0.0	37.897694	13
GCCCACG	1865	0.0	37.878666	18
CACGAGA	1865	0.0	37.878666	21
CGAGACT	1865	0.0	37.878666	23
ACGAGAC	1865	0.0	37.878666	22
AGCCCAC	1880	0.0	37.81578	17
GAGCCCA	1880	0.0	37.81578	16
CGAGCCC	1880	0.0	37.81578	15
CTCCGAG	1875	0.0	37.796635	12
AGACTCG	1870	0.0	37.77738	25
ATCTCGT	1870	0.0	37.77738	37
CGTATGC	1870	0.0	37.77738	41
GATCTCG	1865	0.0	37.75803	36

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content