FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_64.35100000045749.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_64.35100000045749.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259130
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC50291.9407247327596189RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT6190.23887623972523442No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG5490.21186277158183153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6000.041.24858545
ATGCCGT6700.036.9390344
TATGCCG7000.035.03451543
CGTATGC7150.034.29952241
GGATAGA401.550129E-533.768391
CATCTCG7350.033.36620336
GTTATAC7350.033.36620330
TCTCGTA7300.033.2865338
GTATGCC7400.033.1407542
TGTTATA7400.033.1407529
TATACAT7350.033.0600932
CTCGTAT7450.032.91833539
TCGTATG7500.032.6988840
ATCTCGT7450.032.6163337
TACATCT7600.032.2686334
ACTGTTA7650.032.05772427
CGAGACT8000.031.2176823
CTGTTAT7950.030.84828
CGTTACT300.00513600130.004762
GACTGTT8250.029.99897226
Produced by FastQC (version 0.11.2)