FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_63.3510000004572f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_63.3510000004572f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377711
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC75501.9988827436849865RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT10640.2816968528848776No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG7480.1980350055995192No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG6050.16017537217608172No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG5310.14058367376115602No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5020.1329058460039554No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA4640.12284524411520978No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4170.11040186809491914No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4160.11013711541363634No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA3970.10510681446926354No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA3800.1006060188874563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8250.043.09065645
ATGCCGT9100.039.06570444
TATGCCG10100.035.19781543
GTATGCC10300.034.0774742
GTTATAC10400.033.96614530
CGTATGC10350.033.91284641
CTCGTAT10200.033.749839
CATCTCG10200.033.749836
TGTTATA10500.033.6426629
ATACATC10500.033.6426633
TCGTATG10300.033.6405840
TACATCT10500.032.99980534
CGAGACT10850.032.97215723
CTGTTAT10800.032.91647328
ACTGTTA10750.032.8602727
TATACAT10700.032.80354732
TCTCGTA10700.032.1727138
ACGAACA352.816913E-432.1426711
ACGAGAC11200.031.94177622
CCACGAG11200.031.94177620
Produced by FastQC (version 0.11.2)