FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_62.35100000045705.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_62.35100000045705.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352899
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC92842.630781045001544RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT12970.3675272528400477No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG7360.2085582560449307No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG3920.11107994071958266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGA207.022492E-445.0053866
TGCCGTC11650.042.68146545
ATGCCGT13500.036.83252344
CACTATA250.002103898436.0043072
GCCCGCA250.002105362535.99920730
CCCGCAG250.002105362535.99920731
TATGCCG14250.035.0518643
CGTATGC14500.034.29234741
GTATGCC14600.034.21157542
CATCTCG14650.033.9412336
TCGTATG14650.033.9412340
ACGAGAC15000.033.59926222
GCCCACG14950.033.56113418
GTTATAC14850.033.48410830
ACTGTTA14800.033.4452127
TGTTATA14850.033.33259629
CTCGTAT15000.033.29926739
CCCACGA15100.033.22774519
TCTCGTA15050.033.18863738
CGAGACT15050.033.18863723
Produced by FastQC (version 0.11.2)