FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_61.351000000456ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_61.351000000456ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131053
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC19481.4864215241161973RNA PCR Primer, Index 39 (95% over 21bp)
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG2610.19915606662953156No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC1970.15032086255179203No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA1950.14879476242436268No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA1660.12666631057663694No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT1460.11140530930234332No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC1320.10072260841033781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTA1950.040.3825627
TCGTATG1900.040.26111240
TATACAT1900.040.26111232
TATGCCG1850.040.1330943
CGTATGC1950.039.22877541
GTTATAC1950.039.22877530
ATCTCGT1900.039.0769637
ATGCCGT1900.039.0769644
TACCCGG356.1919327E-638.584196
ACCTATA356.1919327E-638.584191
ATACCCG356.1919327E-638.584195
CGTCGGG356.2081926E-638.5694714
TGTTATA2050.038.41267829
GTATGCC1950.038.0749942
ATACATC1950.038.0749933
GCCCACG2100.037.49809318
CATCTCG2050.037.31517436
CACGAGA2150.036.6260421
CCCACGA2150.036.6260419
CGAGACT2150.036.6260423