Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_61.351000000456ea.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 131053 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 1948 | 1.4864215241161973 | RNA PCR Primer, Index 39 (95% over 21bp) |
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG | 261 | 0.19915606662953156 | No Hit |
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC | 197 | 0.15032086255179203 | No Hit |
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA | 195 | 0.14879476242436268 | No Hit |
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA | 166 | 0.12666631057663694 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 146 | 0.11140530930234332 | No Hit |
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC | 132 | 0.10072260841033781 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACTGTTA | 195 | 0.0 | 40.38256 | 27 |
TCGTATG | 190 | 0.0 | 40.261112 | 40 |
TATACAT | 190 | 0.0 | 40.261112 | 32 |
TATGCCG | 185 | 0.0 | 40.13309 | 43 |
CGTATGC | 195 | 0.0 | 39.228775 | 41 |
GTTATAC | 195 | 0.0 | 39.228775 | 30 |
ATCTCGT | 190 | 0.0 | 39.07696 | 37 |
ATGCCGT | 190 | 0.0 | 39.07696 | 44 |
TACCCGG | 35 | 6.1919327E-6 | 38.58419 | 6 |
ACCTATA | 35 | 6.1919327E-6 | 38.58419 | 1 |
ATACCCG | 35 | 6.1919327E-6 | 38.58419 | 5 |
CGTCGGG | 35 | 6.2081926E-6 | 38.56947 | 14 |
TGTTATA | 205 | 0.0 | 38.412678 | 29 |
GTATGCC | 195 | 0.0 | 38.07499 | 42 |
ATACATC | 195 | 0.0 | 38.07499 | 33 |
GCCCACG | 210 | 0.0 | 37.498093 | 18 |
CATCTCG | 205 | 0.0 | 37.315174 | 36 |
CACGAGA | 215 | 0.0 | 36.62604 | 21 |
CCCACGA | 215 | 0.0 | 36.62604 | 19 |
CGAGACT | 215 | 0.0 | 36.62604 | 23 |