FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_60.351000000456c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_60.351000000456c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411127
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC155253.776205406115386RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT24200.5886258990530907No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG10320.2510173255466072No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCC9060.22036986138103312No Hit
CTTTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTGGTAAAT9000.2189104583255296No Hit
ACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAA7390.17974980966951817No Hit
CCCCAATATTTAGCCTTAGATGGCATTTACCACCCATTTTGAGCTGCATTC7380.17950657582693424No Hit
GCTTTGTGATACACTTTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAA7370.17926334198435034No Hit
GTGATACACTTTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGG6700.16296667453122757No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCCCCAATATTTAGCCTTAGATGG6110.1486158778187762No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5950.14472413633743345No Hit
GCTCAAAATGGGTGGTAAATGCCATCTAAGGCTAAATATTGGGGAGAGACC5900.14350796712451383No Hit
GTACTTGTTCGCTATCGGTCTCTCCCCAATATTTAGCCTTAGATGGCATTT5870.14277826559676207No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCC5820.14156209638384246No Hit
CTGTACTTGTTCGCTATCGGTCTCTCCCCAATATTTAGCCTTAGATGGCAT5460.13280567805082127No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT5360.13037333962498204No Hit
TACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA5340.1298868719398142No Hit
CTATCGGTCTCTCCCCAATATTTAGCCTTAGATGGCATTTACCACCCATTT4630.11261726911635578No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC4590.11164433374602008No Hit
ATATTTAGCCTTAGATGGCATTTACCACCCATTTTGAGCTGCATTCCCAAA4510.1096984630053487No Hit
GTTTGGGAATGCAGCTCAAAATGGGTGGTAAATGCCATCTAAGGCTAAATA4180.1016717462000793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC17000.042.6197245
ATGCCGT19700.036.77844244
CGGGAAT250.002105034736.001754
CCCAGCA250.00210629235.997379
TATGCCG21150.034.25698543
GTATGCC21200.034.17619342
CGTATGC21250.034.09577641
CTCGTAT21250.034.0916339
CATCTCG21350.033.82657236
TCGTATG21500.033.80397440
CGAGACT21600.033.54329723
TATACAT21500.033.49000532
GTTATAC21600.033.33495330
CTGTTAT21750.033.31196628
CCCACGA21750.033.30791519
CCACGAG21800.033.23151820
ACTGTTA21750.033.20851527
TCTCGTA21850.033.15547638
TGTTATA21800.033.13234329
ACGAGAC21950.033.0084422
Produced by FastQC (version 0.11.2)