FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_59.351000000456a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_59.351000000456a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381531
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC106472.7905989290516366RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT28820.7553776757327714No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG8190.21466145608089512No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT3820.10012292579108906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11400.043.81719245
ATGCCGT14100.035.4266744
GTTATAC15200.033.30698830
TATGCCG15100.033.08053243
CGTATGC15200.032.86289641
GTATGCC15350.032.68834342
ACTGTTA15600.032.597227
CTCGTAT15350.032.5417639
TCTCGTA15350.032.5417638
CATCTCG15500.032.22261436
CGAGACT15850.032.08304623
TCGTATG15600.032.02025640
CCCACGA15900.031.98215519
CTGTTAT15900.031.98215528
ACGAGAC15900.031.98215522
GCCCACG16000.031.78226918
CACGAGA16150.031.76572221
TATACAT16000.031.6416432
TGTTATA16100.031.58486229
CCACGAG16100.031.58486220
Produced by FastQC (version 0.11.2)