Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_58.3510000004568d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 299446 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 4986 | 1.6650748382012117 | RNA PCR Primer, Index 39 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 1662 | 0.5550249460670705 | No Hit |
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT | 688 | 0.2297576190698824 | No Hit |
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT | 442 | 0.14760591225129072 | No Hit |
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA | 440 | 0.146938012195855 | No Hit |
GGTGAAGACGGTGAGAAGAAGAAGAAGGAGAAAAAGAAGAAGATTGTTGAA | 385 | 0.12857076067137313 | No Hit |
GTTTTCACATGATCTCCTTCAACAATCTTCTTCTTTTTCTCCTTCTTCTTC | 372 | 0.12422941031104105 | No Hit |
GTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACATGATCTCCTTC | 348 | 0.1162146096458126 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG | 305 | 0.10185475845394494 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCCATC | 30 | 2.1620945E-6 | 44.993988 | 7 |
TGCCGTC | 605 | 0.0 | 42.391033 | 45 |
GGCTAGT | 40 | 3.4459117E-7 | 39.376316 | 23 |
AAGCGCC | 40 | 1.5528156E-5 | 33.762405 | 4 |
GGGCTAG | 40 | 1.5581538E-5 | 33.74549 | 22 |
GCCATCC | 40 | 1.5581538E-5 | 33.74549 | 8 |
ATGCCGT | 805 | 0.0 | 31.859097 | 44 |
CGTATGC | 830 | 0.0 | 30.628439 | 41 |
TATGCCG | 830 | 0.0 | 30.628439 | 43 |
CATCTCG | 835 | 0.0 | 30.175608 | 36 |
ATAGGTG | 30 | 0.005132017 | 30.011026 | 3 |
GCGCCAT | 45 | 3.477553E-5 | 30.006014 | 6 |
AGCGCCA | 45 | 3.477553E-5 | 30.006014 | 5 |
AGCATGG | 30 | 0.005144611 | 29.995993 | 10 |
GGGGCTA | 45 | 3.4854955E-5 | 29.99599 | 21 |
CTCGTAT | 850 | 0.0 | 29.90777 | 39 |
GTATGCC | 850 | 0.0 | 29.90777 | 42 |
GTTATAC | 860 | 0.0 | 29.56494 | 30 |
TCGTATG | 865 | 0.0 | 29.389135 | 40 |
TCTCGTA | 860 | 0.0 | 29.298409 | 38 |