FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_58.3510000004568d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_58.3510000004568d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299446
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC49861.6650748382012117RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT16620.5550249460670705No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT6880.2297576190698824No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT4420.14760591225129072No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4400.146938012195855No Hit
GGTGAAGACGGTGAGAAGAAGAAGAAGGAGAAAAAGAAGAAGATTGTTGAA3850.12857076067137313No Hit
GTTTTCACATGATCTCCTTCAACAATCTTCTTCTTTTTCTCCTTCTTCTTC3720.12422941031104105No Hit
GTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACATGATCTCCTTC3480.1162146096458126No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG3050.10185475845394494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCATC302.1620945E-644.9939887
TGCCGTC6050.042.39103345
GGCTAGT403.4459117E-739.37631623
AAGCGCC401.5528156E-533.7624054
GGGCTAG401.5581538E-533.7454922
GCCATCC401.5581538E-533.745498
ATGCCGT8050.031.85909744
CGTATGC8300.030.62843941
TATGCCG8300.030.62843943
CATCTCG8350.030.17560836
ATAGGTG300.00513201730.0110263
GCGCCAT453.477553E-530.0060146
AGCGCCA453.477553E-530.0060145
AGCATGG300.00514461129.99599310
GGGGCTA453.4854955E-529.9959921
CTCGTAT8500.029.9077739
GTATGCC8500.029.9077742
GTTATAC8600.029.5649430
TCGTATG8650.029.38913540
TCTCGTA8600.029.29840938