H23LNBCXX_l01n01_bir_0113_pc_57.35100000045663.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pc_57.35100000045663.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	138239
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	28029	20.27575430956532	RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT	6318	4.570345560948792	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG	1788	1.2934121340576827	No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT	813	0.5881118931705235	RNA PCR Primer, Index 39 (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCTTC	172	0.12442219634111937	RNA PCR Primer, Index 39 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAT	20	7.001463E-4	45.01339	6
TGCCGTC	3005	0.0	42.676125	45
GATAGTC	25	0.0021013778	35.99768	7
ATGCCGT	3685	0.0	34.80102	44
GCCGTCT	160	0.0	33.74783	45
TATGCCG	3910	0.0	32.798405	43
GTATGCC	3930	0.0	32.63149	42
CGTATGC	3925	0.0	32.615738	41
TCGTATG	3925	0.0	32.615738	40
CTCGTAT	3915	0.0	32.58411	39
TCTCGTA	3910	0.0	32.568237	38
ATCTCGT	3915	0.0	32.52664	37
CATCTCG	3925	0.0	32.443775	36
AGCCCAC	3955	0.0	32.42522	17
GCCCACG	3965	0.0	32.34344	18
GAGCCCA	3965	0.0	32.34344	16
CACGAGA	3960	0.0	32.32747	21
CCCACGA	3960	0.0	32.32747	19
CCACGAG	3960	0.0	32.32747	20
CGAGACT	3970	0.0	32.302708	23