FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_56.3510000004564a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_56.3510000004564a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183057
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC39592.1627143458048588No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT10960.5987206170755557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3600.038.7450645
GCCCGGT352.8132857E-432.1387636
ATAGTAG300.00512557430.0125733
ATGCCGT4650.029.99617844
GGCTCAC300.00513929429.99617810
ACTACCG4900.029.39203827
TATGCCG4850.029.22307843
GACTACC5000.028.80419526
GTATGCC4850.028.7592242
CGTATGC4800.028.59010541
CTACCGC5050.028.51900928
CCCACGA5100.028.23169519
CCGCTGA5150.027.957631
AGACTAC5200.027.69634425
TACCGCT5200.027.69634429
ACCGCTG5200.027.68877830
CCACGAG5200.027.68877820
CGCTGAT5150.027.52076332
CGAGACT5250.027.4325723
GCCCACG5250.027.42507618
Produced by FastQC (version 0.11.2)