FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_55.35100000045620.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_55.35100000045620.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344672
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC3724510.8059256336459No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT72062.09068331631232No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG19470.5648848760560765No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT5830.16914631881905115No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5230.15173846439513508No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA5170.1499976789527435No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4830.14013322811252438No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT4790.1389727044842633No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4340.12591681366632626No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT4210.12214511187447775No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC3540.10270634110110483No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATT207.0266303E-444.9997112
TGCCGTC41650.043.86526545
ATGCCGT49900.036.4777244
CACCTAT250.002102136436.0102161
TCATAGG250.002105133435.9997672
ACCCGCT250.002105133435.9997676
TATGCCG52100.034.9805843
GACTACC52700.034.7957926
ACTACCG52650.034.78610227
GTATGCC52400.034.78030842
CTACCGC52600.034.77639428
TACCGCT52600.034.73361629
CGTATGC52350.034.6845941
ACGAGAC52950.034.63150422
ATCTCGT52450.034.6184637
GATCTCG52100.034.59190836
CGCTGAT52150.034.55874332
CCGCTGA52350.034.5556531
CGAGACT52950.034.5465223
GCTGATC52250.034.53566433
Produced by FastQC (version 0.11.2)