FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_54.35100000045606.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_54.35100000045606.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215979
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC23531.0894577713574005No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT4260.19724139846929561No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2380.11019589867533416No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2380.11019589867533416No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGCA207.022512E-444.9974539
GCGGCAC207.022512E-444.9974540
TGCCGTC2900.042.67000245
GGCGTGA250.002101522936.006333
CGACCGG250.002101522936.006327
GGCACTC250.002103913635.99796342
CACTCGG250.002103913635.99796344
GCACTCG250.002103913635.99796343
ATGCCGT3500.035.3551444
TATGCCG3650.033.9021943
GTATGCC3650.033.9021942
CTACCGC3600.033.13079528
ACCGCTG3700.032.84358630
CGTATGC3700.032.8359841
CCCACGA3700.032.8359819
GACTACC3650.032.6769526
TACCGCT3650.032.6769529
GCCCACG3750.032.39816718
CTCGTAT3800.031.97187439
TCTCGTA3800.031.97187438
Produced by FastQC (version 0.11.2)