FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_53.351000000455de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_53.351000000455de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401434
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC52071.297099896869722No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT16130.4018095128962669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6350.042.51650645
ATGCCGT8050.033.53786544
ACTACCG8250.032.18342227
GGGCGCC352.816305E-432.1444621
GGCGCCA352.816305E-432.1444622
CTACCGC8300.031.98954428
GACTACC8350.031.7979926
TATGCCG8500.031.76233143
GTATGCC8500.031.76233142
TACCGCT8450.031.42168429
CGTATGC8650.031.2115441
GCTGATC8550.031.05417833
GCCCACG8800.030.68334818
CGAGACT8750.030.60152623
ACCGCTG8700.030.51876330
CACGAGA8850.030.50999521
CCCACGA8850.030.50999519
TCGTATG8850.030.50619340
GATCTCG8950.030.4167236
CGCTGAT8750.030.3443732
Produced by FastQC (version 0.11.2)