FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_52.351000000455b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_52.351000000455b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432948
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC34160.7890093036577142No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT5970.137891848443693No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5790.13373430527453642No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5300.12241654886961022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4050.039.44140645
TACCCGT403.4536424E-739.3719720
CAGCCCC250.00210526336.00138528
CCAGTAT250.00210526336.00138527
GTGAGCG250.00210645735.9972315
ATGCCGT4700.033.98674844
ACTACCG4750.033.63287427
TACCGCT4750.033.63287429
TATGCCG4800.033.27868743
CTACCGC4850.032.93941528
CGTATGC4900.032.5995341
GACTACC4950.032.27396826
CCCACGA4950.032.2702419
ACCGCTG5000.031.95123330
GCCCACG5100.031.7622618
GTATGCC5050.031.63122742
CACGAGA5150.031.01702921
CGAGACT5200.030.71878823
ACGAGAC5200.030.71878822
GCTGATC5150.030.5801733
Produced by FastQC (version 0.11.2)