FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_51.3510000004559b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_51.3510000004559b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230580
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC45451.9711163153786107No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT6160.26715239829993925No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG2960.12837193165062016No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2830.12273397519299159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5450.040.04471645
ATGCCGT5550.039.3231944
GACTACC5850.037.69121626
CGTATGC5900.037.371841
CTACCGC5900.037.371828
TACCGCT5900.037.371829
TATGCCG5850.037.30661443
GTATGCC6000.036.7489442
CGAGACT6000.036.7489423
ACTACCG6100.036.14649627
ACCGCTG6150.035.8526230
CCCACGA6150.035.8526219
TCGTATG6100.035.77765340
ACGAGAC6300.035.3561222
GCCCACG6250.035.2789818
CTCGTAT6200.035.20059639
GCTGATC6200.034.83770433
CACGAGA6450.034.53388621
GATCTCG6200.034.47480836
TCTCGTA6300.034.28472538