FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_50.35100000045571.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_50.35100000045571.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172445
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC21031.2195192670126707No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT3090.17918756705036387No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2980.17280872162138652No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2610.1513526051784627No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA2010.1165589028385862No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG207.006792E-445.0127681
TGCCGTC2250.039.99974445
ATGCCGT2350.037.34018344
ACAGTAT250.002102221835.9997676
CTTATAG250.002102221835.9997673
TAGGGAG250.002102221835.9997675
ACTACCG2600.035.4805427
ACCGCTG2550.035.2938930
GACTACC2550.035.2938926
TACCGCT2600.034.6151629
GCCCACG2700.034.16644318
CTCGTAT2650.033.96204439
GCTGATC2650.033.96204433
CTACCGC2650.033.96204428
CGTATGC2600.033.74978341
CCCACGA2750.033.5452419
CCGCTGA2700.033.3331231
TCGTATG2700.033.3331240
CGAGACT2700.033.3331223
TATGCCG2650.033.11299543
Produced by FastQC (version 0.11.2)