FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_5.35100000044fd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_5.35100000044fd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376147
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC57081.5174918316509236No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT9220.24511693566610926RNA PCR Primer, Index 44 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6350.038.26669745
ATGCCGT6750.035.99904644
CTAACGC250.002105585535.99904337
TATGCCG7150.033.98510743
CGTATGC7300.033.28678541
GAGACCC7200.033.12412324
GCCCACG7350.033.06034518
CCCACGA7350.033.06034519
GTATGCC7450.032.91858742
CTAGAAT7400.032.53291730
CCCTAGA7300.032.36215228
TCGTATG7450.032.31457540
AGACCCT7400.032.2288725
TAGAATA7550.032.18457431
CACGAGA7650.031.7638621
ACCCTAG7450.031.71056627
CTCGTAT7600.031.67678839
CGTCGGG1000.031.4991614
ACGAGAC7800.031.44147322
TATCTCG7550.031.2905636
Produced by FastQC (version 0.11.2)