FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_49.35100000045558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_49.35100000045558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87177
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC75958.712160317514941No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT9561.0966195212039873No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG2090.23974213381970014No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT1830.20991775353590972No Hit
ATTTGATTCCATTTGATGTTGATTCCATTCGATTCCATTTTATGATGATTC1710.196152654943391No Hit
ATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCTTC1030.11815042958578524TruSeq Adapter, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGA206.99742E-445.022
ATGGCCC206.99742E-445.034
GCCCTGA206.99742E-445.037
CCAAGCA206.99742E-445.027
AGTACAG206.99742E-445.021
TGCCGTC7700.042.95454845
ATGCCGT8600.038.459344
CCCTGAC250.002096463736.038
TGGAGAG250.002096463736.027
AATCACG250.002096463736.035
GGAGAGA250.002096463736.028
AGAACTT250.002096463736.013
AGAGTTC250.002096463736.026
CCGTATG250.002096463736.011
GATGGCC250.002096463736.033
AGTTCCA250.002096463736.028
ACCAAGC250.002096463736.026
GAATTGG250.002096463736.036
TCCTTTG250.002096463736.044
GAGATGG250.002096463736.031
Produced by FastQC (version 0.11.2)