FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_48.3510000004553e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_48.3510000004553e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249547
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC69262.775429077488409RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT19580.7846217345830644No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG4410.1767202170332643No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCC207.0248166E-444.99719625
CGGCTCA207.0248166E-444.99719627
TGCCGTC8250.043.08822345
GCCCCTA250.00210459935.99775327
CCCCTAT250.00210459935.99775328
ACGGCTC250.00210459935.99775326
CGCCCCT250.00210459935.99775326
ATGCCGT10100.035.19582444
TATGCCG10750.033.06770743
GTATGCC10750.033.06770742
ACCTGTG10700.032.80169327
CGTATGC10900.032.61264441
CATCTCG10950.032.4637336
TCGTATG10950.032.4637340
GTTATAT352.8019014E-432.1666371
CTCGTAT11150.032.08320239
TCTCGTA11150.032.08320238
GGACATC11000.031.90709933
CGAGACC11300.031.8564223
TGTGGAC10950.031.8473330
Produced by FastQC (version 0.11.2)