FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_46.351000000454f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_46.351000000454f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323857
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC214216.614339044701828RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT45531.405867404440849No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA10560.3260698394661842No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10230.31588015698286587No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC9600.2964271267874401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA9200.2840759965046301No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC7010.21645355820624534No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC6690.20657265397999733No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG6390.19730930626788984No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA6360.1963829714966791No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA6040.1865020672704311No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG5970.18434061947093933No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT5090.15716813284875733No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4980.15377157202098457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG4330.1337009853114183No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4330.1337009853114183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.1330834287972778No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3990.12320252457102981No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG3920.12104107677153805No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3780.11671818117255454No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT3360.10374949437560405No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC3330.10282315960439331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCG3300.10189682483318255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTCG207.026131E-444.9995421
TGCCGTC23250.043.25761845
GGGTACG250.002104985335.9996339
ATGCCGT28600.035.1657244
TATGCCG29400.034.132343
CGTATGC29450.034.07435241
TCGTATG29450.033.9979540
TGTGGAC29150.033.96191430
GTATGCC29550.033.9590442
GGACATC29200.033.90376333
ATCTCGT29400.033.90271437
CATCTCG29350.033.8838136
TCTCGTA29500.033.8640638
GTGGACA29250.033.84580631
CTCGTAT29450.033.84515439
CCTGTGG29450.033.76875328
CTGTGGA29500.033.71151729
ACGAGAC29950.033.65574622
CGAGACC29950.033.5806223
ACCTGTG29700.033.48450527
Produced by FastQC (version 0.11.2)