FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_45.351000000454df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_45.351000000454df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341140
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC75612.2163920970862403RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT33010.9676379199155772No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG6020.17646713959078383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8100.041.9426745
CGCCACG301.1385231E-437.49841320
CAACCGC352.8169472E-432.14149516
GTCCCGG352.8169472E-432.14149526
ATGCCGT11150.030.46956344
GTAATTG300.00513225630.0119291
TTAGGCG300.005143308529.99873227
CACGCCG300.005143308529.99873220
TCGTGAG300.005143308529.99873227
AACCGCC453.484732E-529.9987317
TATGCCG11500.029.54222743
GTATGCC11700.029.03723342
ACCGCGA554.1550265E-628.6351538
TACCGCG554.1550265E-628.6351537
GCGAGGT554.1550265E-628.63515311
CGTATGC11950.028.42975841
ACCTGTG11800.028.40981327
CGAGACC12150.028.14695723
CTAACGT406.148155E-428.1320573
CGGTTCC406.158711E-428.1238130
Produced by FastQC (version 0.11.2)