H23LNBCXX_l01n01_bir_0113_pc_44.351000000454b5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pc_44.351000000454b5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359009
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC	8656	2.411081616338309	RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT	1955	0.5445545933388857	No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA	809	0.22534254015916036	No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT	751	0.20918695631585837	No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG	707	0.19693099615887064	No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA	507	0.1412220863543811	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	434	0.1208883342757424	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	368	0.10250439404026082	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	20	7.0280844E-4	44.99833	5
TGCCGTC	960	0.0	42.180058	45
CTAGACT	25	0.0021055664	35.99866	4
ACTAGAC	25	0.0021055664	35.99866	3
GTTCTAG	65	3.6379788E-10	34.633392	1
ATGCCGT	1175	0.0	34.462006	44
CGTATGC	1200	0.0	33.37376	41
ACCTGTG	1200	0.0	33.19089	27
TATGCCG	1215	0.0	33.146915	43
GTATGCC	1215	0.0	33.146915	42
CATCTCG	1210	0.0	33.097946	36
TCTCGTA	1205	0.0	33.048565	38
CTCGTAT	1210	0.0	32.912003	39
CGAGACC	1235	0.0	32.796875	23
GGACATC	1205	0.0	32.679688	33
TGTGGAC	1200	0.0	32.628334	30
TCGTATG	1225	0.0	32.508995	40
CACGAGA	1250	0.0	32.403313	21
TGGACAT	1225	0.0	32.146137	32
GCCGTCT	35	2.8194574E-4	32.137184	45