Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_43.3510000004549c.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 307787 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC | 4701 | 1.5273549565121334 | RNA PCR Primer, Index 20 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT | 522 | 0.16959780627511883 | No Hit |
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA | 320 | 0.10396800384681615 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 645 | 0.0 | 39.069626 | 43 |
ATGCCGT | 645 | 0.0 | 39.069626 | 44 |
CGTATGC | 650 | 0.0 | 38.769093 | 41 |
GTATGCC | 660 | 0.0 | 38.181683 | 42 |
CACGAGA | 665 | 0.0 | 37.8946 | 21 |
TCGTATG | 665 | 0.0 | 37.8946 | 40 |
GACATCT | 655 | 0.0 | 37.786125 | 34 |
GTGGACA | 665 | 0.0 | 37.556255 | 31 |
GGACATC | 660 | 0.0 | 37.499866 | 33 |
CCCACGA | 675 | 0.0 | 37.3332 | 19 |
TGGACAT | 670 | 0.0 | 37.275986 | 32 |
CGAGACC | 680 | 0.0 | 37.058693 | 23 |
CTCGTAT | 675 | 0.0 | 36.999866 | 39 |
ACGAGAC | 690 | 0.0 | 36.847694 | 22 |
TGCCGTC | 685 | 0.0 | 36.78819 | 45 |
TGTGGAC | 680 | 0.0 | 36.72781 | 30 |
TCTCGTA | 680 | 0.0 | 36.72781 | 38 |
ACCTGTG | 695 | 0.0 | 36.582603 | 27 |
GCCCACG | 690 | 0.0 | 36.52161 | 18 |
ATCTCGT | 685 | 0.0 | 36.459724 | 37 |