FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_43.3510000004549c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_43.3510000004549c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307787
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC47011.5273549565121334RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT5220.16959780627511883No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3200.10396800384681615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6450.039.06962643
ATGCCGT6450.039.06962644
CGTATGC6500.038.76909341
GTATGCC6600.038.18168342
CACGAGA6650.037.894621
TCGTATG6650.037.894640
GACATCT6550.037.78612534
GTGGACA6650.037.55625531
GGACATC6600.037.49986633
CCCACGA6750.037.333219
TGGACAT6700.037.27598632
CGAGACC6800.037.05869323
CTCGTAT6750.036.99986639
ACGAGAC6900.036.84769422
TGCCGTC6850.036.7881945
TGTGGAC6800.036.7278130
TCTCGTA6800.036.7278138
ACCTGTG6950.036.58260327
GCCCACG6900.036.5216118
ATCTCGT6850.036.45972437