FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_42.35100000045472.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_42.35100000045472.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296856
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC39771.3397067938663865RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT12480.42040585334303504No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3250.1094806909747487No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG3250.1094806909747487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4150.042.82908245
CGGTCCA301.1382339E-437.49803528
CGCGTCG301.1382339E-437.49803523
CATACCG250.002101659536.0102425
AGGCCGC352.8106742E-432.1520046
TAGGCCG352.8106742E-432.1520045
ACGCCCC352.816238E-432.1411734
ATCCGCG352.816238E-432.1411720
CGATCGG352.816238E-432.1411745
CCACGCC352.816238E-432.1411732
ATGCCGT5700.031.18257744
ACACTAT453.4754572E-530.0085376
TATGCCG5950.029.87238543
TTAGGGA702.8630893E-828.9368044
GTATGCC6150.028.90092542
CGTATGC6200.028.66785441
CTCGTAT6200.028.66785439
TGTGGAC6300.028.56993130
ACCTGTG6250.028.43850927
CGAGACC6250.028.43850923