Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_42.35100000045472.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 296856 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC | 3977 | 1.3397067938663865 | RNA PCR Primer, Index 20 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT | 1248 | 0.42040585334303504 | No Hit |
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA | 325 | 0.1094806909747487 | No Hit |
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG | 325 | 0.1094806909747487 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 415 | 0.0 | 42.829082 | 45 |
CGGTCCA | 30 | 1.1382339E-4 | 37.498035 | 28 |
CGCGTCG | 30 | 1.1382339E-4 | 37.498035 | 23 |
CATACCG | 25 | 0.0021016595 | 36.010242 | 5 |
AGGCCGC | 35 | 2.8106742E-4 | 32.152004 | 6 |
TAGGCCG | 35 | 2.8106742E-4 | 32.152004 | 5 |
ACGCCCC | 35 | 2.816238E-4 | 32.14117 | 34 |
ATCCGCG | 35 | 2.816238E-4 | 32.14117 | 20 |
CGATCGG | 35 | 2.816238E-4 | 32.14117 | 45 |
CCACGCC | 35 | 2.816238E-4 | 32.14117 | 32 |
ATGCCGT | 570 | 0.0 | 31.182577 | 44 |
ACACTAT | 45 | 3.4754572E-5 | 30.008537 | 6 |
TATGCCG | 595 | 0.0 | 29.872385 | 43 |
TTAGGGA | 70 | 2.8630893E-8 | 28.936804 | 4 |
GTATGCC | 615 | 0.0 | 28.900925 | 42 |
CGTATGC | 620 | 0.0 | 28.667854 | 41 |
CTCGTAT | 620 | 0.0 | 28.667854 | 39 |
TGTGGAC | 630 | 0.0 | 28.569931 | 30 |
ACCTGTG | 625 | 0.0 | 28.438509 | 27 |
CGAGACC | 625 | 0.0 | 28.438509 | 23 |