FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_41.35100000045459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_41.35100000045459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152866
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC1914912.52665733387411RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT52653.44419295330551No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG9080.5939842738084335No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT4050.2649379194850392No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT253.8773953E-544.9950941
TGCCGTC20850.042.4054545
GGGAGGT250.002099159936.0078525
GGGTCTA250.002102535235.99607516
GCCGTCT1050.034.2819845
ATGCCGT26700.033.11436544
TATAATC352.810823E-432.139352
CTCGTAT27600.032.11606239
ATCTCGT27600.032.0345537
CATCTCG27650.031.97662236
TCTCGTA27650.031.97662238
TATGCCG27800.031.9659443
CGTATGC27750.031.94246141
GCCCACG27850.031.90854818
GAGCCCA27850.031.90854816
CACGAGA27950.031.88530721
GGACATC27750.031.87181533
AGCCCAC27900.031.85136617
CCACGAG27900.031.85136620
CCTGTGG27700.031.84809928
Produced by FastQC (version 0.11.2)