FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_40.3510000004543f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_40.3510000004543f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232827
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC10040.4312214648644702No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4360.18726350466225997No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT4340.1864044977601395No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA3690.1584867734412246No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT3280.1408771319477552No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT2380.10222182135233455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1500.035.99707843
ATGCCGT1500.035.99707844
TGCCGTC1500.035.99707845
GCCCACG1550.034.8358818
CCCACGA1500.034.497219
ATACCCG401.5515388E-533.7617655
CGTATGC1600.033.7472641
GTATGCC1650.032.72461742
AGACAGG1700.031.76212725
CGCGCGC453.480949E-529.99756610
TCGCGCG453.480949E-529.9975669
CGAGACA1800.028.74766723
CTCGTAT1900.028.41874739
CTTAGAC406.1310176E-428.140853
TACCCGG406.138732E-428.1348026
GGGGCAA406.154184E-428.12271718
GACAGCG654.848753E-727.6900627
CGCGCTT654.848753E-727.69006212
GACAGGA1950.027.6900626
CACGAGA1900.027.23463221