FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_4.35100000044fbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_4.35100000044fbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344607
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC50461.464276697803585No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT8720.253041870884805RNA PCR Primer, Index 44 (95% over 21bp)
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5740.1665665526237134No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5560.16134321125223808No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA5090.14770448656005247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6300.039.99677745
ACGTGTG301.1378313E-437.50242230
TCGCGCG301.13880385E-437.4969759
GTATGCC6950.035.93235842
ATGCCGT6950.035.93235844
CGTATGC7050.035.42267641
TATGCCG7050.035.42267643
CGAGACC7200.035.30965423
TATCTCG7000.035.0328936
TCGTATG7200.034.68470440
ACCCTAG7350.034.5940727
GACCCTA7300.034.52277826
CCCACGA7300.034.51776519
CACGAGA7450.034.42675421
AGACCCT7400.034.36032525
GCCCACG7350.034.2829518
CTCGTAT7250.034.13517839
GAGACCC7500.033.89726624
CTAGAAT7500.033.30214730
CCCTAGA7500.033.30214728
Produced by FastQC (version 0.11.2)